Fig. 6

Microglia number and morphology, astrocytic GFAP expression and neuronal Map2 intensity in SynIIKO mice after 1 month of voluntary running before seizure onset. All stainings were evaluated in coronal brain sections, and areas included in the analyses are highlighted in purple (a). Photomicrograph of a haematoxylin/eosin staining shows gross histology of the dentate gyrus in the hippocampus of SynIIKO mice at the age of 2 months (group D) (b). Representative immunohistochemical images of Iba1⁺ and GFAP⁺ cell distribution (white arrows) in sedentary (c, e) and exercised (d, f) SynII KO mice, respectively. Haematoxylin/eosin staining of entorhinal cortex with the six cortical layers indicated in roman numbers (g). Map2 immunohistochemical staining of entorhinal cortex in sedentary (h) and exercised (i) SynIIKO mice. Representative Map2⁺ neuronal fibres in entorhinal cortical layer II are indicated by arrows (h-i). Numbers of Iba1⁺ microglial cells in the dentate hilus, granule cell layer (GCL) and molecular layer (ML) of the hippocampus in sedentary and exercised SynIIKO mice (j). Percentage of ramified (RAM), intermediate (INTER) and amoeboid/round (A/R) morphologies of Iba1⁺ cells in the hilus (k), GCL (granule cell layer (l)) and ML (molecular layer (m)). Intensity measurements of GFAP immunohistochemical staining in astrocytic processes within the hilus, GCL and ML of the hippocampus (n)