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Fig. 2 | Sports Medicine - Open

Fig. 2

From: Age-Markers on the Red Blood Cell Surface and Erythrocyte Microparticles may Constitute a Multi-parametric Strategy for Detection of Autologous Blood Transfusion

Fig. 2

Changes of Band 3 (protein and/or complex) and of GYPA concentrations as a function of storage time. a Band 3 levels measured by flow cytofluorimetry; b Band 3 levels measured by western immunoblotting assay; c Band 3 complex levels measured by flow cytofluorimetry analysis, using Eosin-5’-maleimide dye, which binds the whole macro-complex and not only the single protein. d Band 3 protein levels measured by western immunoblotting assay in RBCs fractions isolated by Percoll density gradients; e GYPA levels measured by flow cytofluorimetry f GYPA/Band 3, both measured by flow cytofluorimetry

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