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Sports Medicine - Open
Fig. 1 | Sports Medicine - Open

Fig. 1

From: Age-Markers on the Red Blood Cell Surface and Erythrocyte Microparticles may Constitute a Multi-parametric Strategy for Detection of Autologous Blood Transfusion

Fig. 1

Flow cytofluorimetry gate settings for red blood cells (RBC) microparticles (RMPs) analysis. a Setting of a gate based on cell dimension (SS) and complexity (FS), which allows to include into the analysis only cell ≤ 1 μm. b Setting of a gate based on FL2 detector and cell binding to Glycophorin-A (GYPA)-PE antibody. c Setting of gates based on GYPA expression and dimension. Gate C defines RBCs, gate H identifies the Transitional Events (TE), while gate I identifies events expressing GYPA smaller than 1 nm, representing RMPs

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