Screening of Potential Stress Biomarkers in Sweat Associated with Sports Training

Sports Medicine - Open

Table 2 Identified biomarkers. Biomarker, retention time (rt), ESI operation mode, precursor ion (m/Z) and product fragment ions (m/z), signal (NL), collision energy (eV) and references for identification (Ref.)

Biomarker	rt	Mode	Precursor ion (m/Z)	Fragment ions (m/Z)	Signal	Collision energy (eV)	Ref
Major NTs
Ach	1.59	+	146	60/87	10³	10/19	[24]
Biological amines and metabolites
DA	1.59	+	154	91/137	10²	4	[24, 25]
DOPAC (DA Met)	2.34	-	167	122	10²	10	[24, 25]
3-MT (DA Met)	1.59	+	168	121	10⁴	10	[24, 25]
HVA (DA Met)	2.69	-	181	122	10²	10	[24, 25]
E	1.59	+	184.1	106/166	10³	12/21	[24, 25]
NE	2.56	+	170	107/135	10²	24/15	St
5-HT	1.37	+	177	115/160	10³	4	[24, 25]
5-HIAA (5-HT Met)	1.58	-	190	146	10³	13	[24, 25]
Amino acids
Glu	1.57	+	148	84/130	10⁵	10	[17, 27]
Purines
Ade	1.59	+	268	136/170	10³	12	[28]
Other biomarkers
Amino acid precursors of biological amines
Phe	1.59	+	166.1	77/103/120/149	10⁷	10	St, [17]
Trp	1.64	+	205.2	113/144/159/188/245	10⁶	10	St, [17]
Tyr	1.59	+	182	105/119/123/136/165	10⁶	10	St, [17]
Amino acids
Crea	1.59	+	132.1	43.3/90.2	10⁴	10	[29]
Gln	1.32	+	147	84/85/103/121/130	10⁵	10	[17]
His	1.32	+	156	56/83/93/95/110	10⁵	10	St, [17]
Ile	1.59	+	132	69	10⁵	10	[28]
Leu	1.59	+	132.1	86	10⁷	10	[17]
Lys	1.05	+	147.2	56/84/130	10⁵	10	St
Carboxylic acids
Asc	1.99	-	175	87/115	10³	23/15	St, [30]
Lacta	1.58	-	89	43	10⁶	10	[27, 31]
Carbohydrates
Gluc	1.58	-	179	71/89	10⁵	10	[32]
Breakdown product
Creat	1.58	+	114	44.3/86	10³	10	[29]
Steroid hormones
Cor	1.59	+	363.1	121/309/327	10⁴	10	[11, 23]
Cort	1.59	+	361	163/343	10⁴	10	[23]