Table 1 LC-MSMS operational conditions

UHPLC pre-column

UHPLC column

Security Guard^TM Ultra Holder (AJO-9000 phenomenex®)

Accurore^TM RP-MS Column (2.6 μm, 150 × 2.1 mm, ThermoFisher Scientific)

Column temperature

25 °C

Flow rate

0.25 ml min^-1

Mobile phase

(A) H₂O: 0.1% formic acid (V/V)

(B) Acetonitrile

Equilibration: B: 20% (5 min)

Elution B: 20% (0 min); 90% (1 to 6 min); 80% (7 min); 50% (7.5 min); 20% (8 to 20 min)

Injection volume

10 μl

Ionization Source Analysis mode

Ion-spray voltage

Vaporizer temperature

Capillary temperature

ESI positive and/or negative

Full scan (Q3) and selective reaction monitoring (SRM)

Positive (3500 V) and negative (3500 V)

320 °C

325 °C