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Table 2 PCR conditions

From: Liver-Metabolizing Genes and Their Relationship to the Performance of Elite Spanish Male Endurance Athletes; a Prospective Transversal Study

CYP2D6 Initial denaturation94° 5 min
× 30 cyclesDenaturation94° 1 min
Annealing60° 1 min
Extension72° 2 min
 Final extension72° 5 min
GSTM1 Initial denaturation95° 5 min
× 40 cyclesDenaturation94° 30 sec
Annealing58° 30 sec
Extension72° 45 sec
 Final extension72° 8 min
GSTP Initial denaturation94° 5 min
× 35 cyclesDenaturation94° 30 sec
Annealing55° 30 sec
Extension72° 30 sec
 Final extension72° 5 min
GSTT Initial denaturation95° 5 min
× 30 cyclesDenaturation95° 1 min
Annealing60° 1 min
Extension72° 1 min
 Final extension72° 10 min